FastQCFastQC Report
Wed 25 May 2016
SRR1779977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232090
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT143406.178637597483735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126545.452195269076651No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT84023.6201473566288938No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG22810.9828083932957042No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC16030.6906803395234608No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC7580.3265974406480245No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT6780.29212805377224355No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT6670.28738851307682367TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT3970.17105433237106293No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC3340.14390969020638544No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT2790.12021198672928605No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGACGT2580.11116377267439356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACAA150.002224371569.9864347
ATTATAC19350.063.670621
AGGGGGG26200.062.1196670
CGTCTTA509.767973E-1056.00120548
AATATCG252.3893366E-455.9891436
CCGTCTT34350.053.38120347
CGTCTTC34700.053.25763348
GCCGTCT34650.053.1210146
TCTTCTG34000.052.70701650
GTCTTCT34050.052.6296249
CATTCGC200.0069385752.51244719
CGATGGA200.00695041152.4898236
ATGCCGT36000.052.4898244
TATGCCG36400.052.3936943
TGCCGTC36000.052.29541445
GTATGCC36650.052.22725342
CGTATGC36800.052.1094641
TCGTATG37750.052.0031640
CTTGAAA36000.051.9177957
AATCTCG1350.051.84179736
Produced by FastQC (version 0.11.5)