FastQCFastQC Report
Wed 25 May 2016
SRR1779976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95277
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4585748.130188817867904No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG1207312.671473702992328No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT14111.48094503395363No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC13761.444210040198579No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG12631.3256084889322712No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG4570.4796540613159524No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG3050.3201192312940164No Hit
TATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGT1980.20781510752857457No Hit
CTTTACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG1320.13854340501904971No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1210.12699812126746224No Hit
CTTAATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT1150.12070069376659634No Hit
CTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT1150.12070069376659634No Hit
TCTTAATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1130.1186015512663077No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAAGC150.002218628370.07
GCCTTAC150.002218628370.026
GCAAGCC150.002218628370.08
GAACTAG150.002218628370.030
TGAAAGC150.002218628370.033
TGTGCTA150.002218628370.064
CGCTGGA150.002218628370.02
AAAAATC150.002218628370.012
GTGTGCG150.002218628370.02
GCCGAAT150.002218628370.054
CTAGTAA150.002218628370.068
AAGCAAG150.002218628370.036
AAACCTC150.002218628370.06
TCGCTAA150.002218628370.015
CCGAGCG150.002218628370.024
TTGAACA150.002218628370.04
CTCGCTA150.002218628370.014
GCTAGTA150.002218628370.067
GTATCAG207.891403E-570.057
AGCCTCG150.002218628370.011
Produced by FastQC (version 0.11.5)