FastQCFastQC Report
Wed 25 May 2016
SRR1779976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95277
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT2074521.773355584243838No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2008421.079588987898443No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT1211012.710307839247667No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG31893.3470827167102235No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC24532.574598276604007No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC11691.2269487914187054No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT8220.8627475676186278RNA PCR Primer, Index 36 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC5830.6119000388341362No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT3040.31906966004387205No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT2760.2896816650398312No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC2140.22460824753088365No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAACCCATCTCGTATGCCGT1800.18892282502597688No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT1710.17947668377467804No Hit
TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC1430.1500886887706372No Hit
TATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCT1320.13854340501904971No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT1180.12384940751702929No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG1160.12175026501674066No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCAGT1040.10915541001500888No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG1030.10810583876486456No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC980.10285798251414298No Hit
CTCTTATACACATCTCCGCGCCCACGAGACGGTAAACCATCTCGTATGCC980.10285798251414298No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAACCCATCTCGTATGCCGT960.10075884001385434No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC960.10075884001385434No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCG960.10075884001385434No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCTC150.002214733770.0309931
TCTGGTG150.002214733770.0309926
AGAGAGT150.002214733770.0309927
GATTTTT207.874105E-570.0309828
GCACAGA207.874105E-570.0309823
ATCTCGA252.8224076E-669.9942337
TCTCGAA252.8224076E-669.9942338
CCCTCTT150.00221935469.99422538
TTTACAC207.8946294E-569.9942252
TTAGCGA150.00221935469.99422570
TAGGTTA150.00221935469.99422542
AACTTAT150.00221935469.9942251
TTACTAG150.00221935469.99422517
AGTGGGG150.00221935469.99422551
ATCACGT301.0148142E-769.99422537
ACTAGCA150.00221935469.99422519
GAGAACG150.00221935469.9942257
TCGCTGC207.8946294E-569.9942259
ACGTGGT150.00221935469.99422511
GAACGTG150.00221935469.9942259
Produced by FastQC (version 0.11.5)