FastQCFastQC Report
Wed 25 May 2016
SRR1779975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553537
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG546009.863839273616758No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG108981.9687934139903926No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG17270.311993597537292No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC14440.2608678371996813No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT12210.2205814606792319No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAT200.006950142352.523
GCCGTAT19450.045.88688754
TGGTCGC19950.045.0877249
TCGCCGT19800.045.07575652
CTCGGTG20100.044.92537344
TCGGTGG20200.044.87623645
CGCCGTA19850.044.78589653
CGGTGGT20350.044.54545646
TCTCGGT20150.044.466543
CGTATCA20000.044.4556
GTCGCCG20250.044.4197551
GTAGATC20500.044.21951338
GATCTCG20300.044.1379341
ATCTCGG20350.044.02948442
GTGGTCG20600.043.8349548
CCGTATC20550.043.2603455
AGATCTC20750.043.18072540
TAGATCT20750.043.18072539
GGTGGTC20950.043.10262347
GTATCAT20700.042.94685757
Produced by FastQC (version 0.11.5)