FastQCFastQC Report
Wed 25 May 2016
SRR1779975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553537
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT241444.361768048025697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189683.426690537398584No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT121682.198227038120306No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG29950.5410659088733003No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC22230.40159917042582516No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT13800.2493058277947093RNA PCR Primer, Index 36 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC8260.1492221838829202No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC7100.12826604183640838No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAG150.002223746670.0043329
ATTATAC33600.062.8107151
AGGGGGG41850.060.21334570
CGTATGA1550.056.45001241
CCGTCTT53350.053.5317547
TATGCCG56200.053.37036543
GCCGTCT54350.053.25515746
CGTCTTC54550.053.18822548
CTCGTAT57200.053.04919439
ATGCCGT55800.052.93756544
TCGTATG58200.052.85932540
ATCTCGT56400.052.74672737
TCTCGTA56900.052.7137938
CGTATGC57450.052.69650341
GTATGCC57000.052.6213142
TGCCGTC55900.052.59242645
CGACGTA200.00695092252.4985126
CGTCTTA608.367351E-1152.49851248
ACGACGT200.00695092252.4985125
AATCTCG57000.052.191536