FastQCFastQC Report
Wed 25 May 2016
SRR1779974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114495
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4544439.69081619284685No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG88087.692912354251277No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC10450.9127036115114198No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT10190.8899951962967815No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG8720.7616053102755579No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG2970.25939997379798246No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG2450.21398314336870608No Hit
TATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGT1740.15197170182104022No Hit
TCTTAATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1260.1100484737324774No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCAG150.002216313570.02753432
GTGTCGG252.8254035E-669.9969542
CCTTCAA207.8999095E-569.9969415
TGTGCGC150.0022201669.996943
CTAACTT150.0022201669.9969421
CCGTATT150.0022201669.9969460
CCAAGAG207.8999095E-569.996948
GACGATC150.0022201669.9969423
GCTAACT150.0022201669.9969420
CCGTTGG150.0022201669.996941
AGAGCCT207.8999095E-569.9969411
CTGGGGG352.9530747E-759.99737518
GGTGTCG308.334338E-658.33078841
CAAGTGT252.377292E-456.02202633
AAGTTGC252.377292E-456.02202632
TACTTAA252.3824343E-455.99755541
AACTTAG252.3824343E-455.99755523
CTAAGGA252.3824343E-455.99755560
ATACTTA252.3824343E-455.99755540
TCGGCGG252.3824343E-455.99755552