FastQCFastQC Report
Wed 25 May 2016
SRR1779974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114495
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT1785215.591947246604656Illumina PCR Primer Index 1 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1483812.95951788287698No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT1194910.436263592296607Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG32922.8752347264072666No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC14111.2323682256867112No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT12561.0969911349840604Illumina PCR Primer Index 1 (95% over 24bp)
ATCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC9300.8122625442159047No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC7390.6454430324468318No Hit
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC3540.3091838071531508Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT3510.3065636053976156Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT3290.287348792523691TruSeq Adapter, Index 1 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT2660.23232455565745228No Hit
ATTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT2550.22271714922048996Illumina PCR Primer Index 1 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC2050.17904711996157038Illumina PCR Primer Index 1 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT1710.14935150006550504Illumina PCR Primer Index 1 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC1560.13625049128782918No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG1440.12576968426568846No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATATCGTATGCCGT1420.12402288309533167No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGCTCTCGTATGCCGT1310.11441547665836935No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC1250.109175073147299Illumina PCR Primer Index 1 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGACGT1240.10830167256212062No Hit
ATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC1220.10655487139176383Illumina PCR Primer Index 1 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCCAACACGCTCTCGTATGCCGT1150.1004410672955151No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA150.00221362270.0489728
ACGCAAG150.00221362270.0489730
GCACCGT150.00221362270.0489721
CGCAAGC150.00221362270.0489731
CCACACG207.870865E-570.04896530
GCAACCG150.002221314269.9877736
ATGTGAC150.002221314269.987774
AAAAGCG150.002221314269.9877767
ATCCATA150.002221314269.9877744
GCATCCA150.002221314269.9877742
ATTATAC15800.067.331
TCTTTAT1400.062.4890751
TCGAATG453.8016879E-1062.21135340
AGGGGGG35350.061.67239470
CGAATGC401.0571966E-861.23929641
AACAAGA352.9379225E-760.04197331
ATCTTAT1300.059.2204131
AAACGAT308.297668E-658.3741432
ACAAGAT308.297668E-658.3741432
CGTCTTA308.340814E-658.32314348
Produced by FastQC (version 0.11.5)