FastQCFastQC Report
Wed 25 May 2016
SRR1779973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327932
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4983615.197053047583037No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG99803.0433138577509973No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC14770.45039825329641514No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG12400.37812717270653673No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT10350.31561421270263346No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG3880.11831721210494858No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAT150.002222675670.0064135
CACCGGA150.002222675670.0064116
GTGAACG150.002222675670.0064149
GAACGGT207.9165926E-570.006434
GGACGGT150.002224020469.99573511
CCGCGGA150.002224020469.9957359
TTCGTCG207.922576E-569.9957320
CGCCTTG207.922576E-569.9957325
TCGTCGC207.922576E-569.9957321
GGTGCGT252.3874291E-456.00512713
TGCGTTT252.3874291E-456.00512715
CGACGTA200.006945115552.50480353
TGTACCA200.006945115552.50480338
TCTCTAG200.006949305552.49679658
CGGTGTT200.006949305552.4967969
ATTGCGG200.006949305552.4967965
GTTGGCG200.006949305552.49679631
GCCGTAT16300.047.23638554
TGGTCGC16650.046.88116549
GGCAGCG305.87499E-446.66382258