FastQCFastQC Report
Wed 25 May 2016
SRR1779973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327932
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT207426.325091787321761No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172695.266030762475148No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT131684.015466621128771No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG34641.056316553431809No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC23050.7028896234585219No Hit
ATCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC14630.446129075540051No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT11130.3393996316309479TruSeq Adapter, Index 25 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC8770.26743349230938124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTACG150.002222752270.00581
CGACGAA207.922916E-569.99512522
CGTGCGA150.002224097369.99512511
ACCGTTC150.002224097369.9951258
AGGGGGG37700.061.3617770
ATTATAC16300.060.557161
CAAGGCG308.362689E-658.33816517
GCGACGA252.3893316E-455.99609821
CCGTTCT252.3893316E-455.9960989
ACAGTCG200.006945353552.50434555
TCGATCA200.006945353552.50434515
ATCCACG200.006945353552.5043451
CTACCGA200.006945353552.50434518
GTATACG200.006945353552.50434561
CGAGAAT200.006945353552.50434515
TATACGG200.006945353552.50434562
AGCCGTC608.367351E-1152.4963445
AGATATC608.367351E-1152.4963433
GGTCGGT200.00694954452.4963411
CGTCTAC407.47139E-752.4963448
Produced by FastQC (version 0.11.5)