FastQCFastQC Report
Wed 25 May 2016
SRR1779972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7200
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC6909.583333333333334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2944.083333333333333No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG821.1388888888888888No Hit
TATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGT721.0No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG360.5No Hit
ACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGC330.4583333333333333Illumina Single End PCR Primer 1 (100% over 21bp)
CTTTACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG330.4583333333333333No Hit
TCTTAATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG310.4305555555555556No Hit
CTTAATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT220.3055555555555556No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG210.2916666666666667No Hit
TCTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG140.19444444444444445No Hit
CTTATATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG140.19444444444444445No Hit
CTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT140.19444444444444445No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGAGGTC100.1388888888888889No Hit
CCTGTGTGTAGCACAGAGCTGGGTCTGGTTTTTATAATCCATTAAGTCAC90.125No Hit
TCTCATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT90.125No Hit
GTAATGTTGATAGTAGAATGATGGCTAGGGTGACTTCATATGAGATTGTT80.1111111111111111No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA150.00213594270.049
GTCGACG150.00213594270.048
GACGAAT150.00213594270.051
AGGTCGA150.00213594270.046
CGACGAA150.00213594270.050
GGTCGAC150.00213594270.047
TCTTTAT252.2345094E-455.9999961
AAGGGGG406.4911546E-752.570
TTTATAC200.006676138452.53
CGATCTA1500.035.026
TAGATCT1750.034.036
GTAGATC1650.033.93939235
GATCTCG1650.033.93939238
TGTAGAT1650.033.93939234
TCTAGTG1550.033.87096829
GCGATCT1450.033.79310225
AGATCTC1700.032.94117737
CTAGTGT1600.032.812530
ATCTAGT1500.032.66666828
GCCGTAT1500.032.66666851
Produced by FastQC (version 0.11.5)