FastQCFastQC Report
Wed 25 May 2016
SRR1779972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7200
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG366650.916666666666664No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG570.7916666666666667No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGG550.7638888888888888No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT410.5694444444444444No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT240.33333333333333337No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGG230.3194444444444445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGG220.3055555555555556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGG200.2777777777777778No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG170.2361111111111111No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGG160.2222222222222222No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGG160.2222222222222222No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGG150.20833333333333334No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT150.20833333333333334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG140.19444444444444445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGG140.19444444444444445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG130.18055555555555555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGG130.18055555555555555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGG130.18055555555555555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG120.16666666666666669No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGC120.16666666666666669No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG120.16666666666666669No Hit
GGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110.1527777777777778No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG110.1527777777777778No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.1388888888888889No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGG100.1388888888888889No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGG100.1388888888888889No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT100.1388888888888889Illumina PCR Primer Index 6 (95% over 22bp)
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100.1388888888888889No Hit
GGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGG100.1388888888888889No Hit
GCAGCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCAC100.1388888888888889No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGG100.1388888888888889No Hit
GGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGG90.125No Hit
GGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGG90.125No Hit
GGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGG90.125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGG90.125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG90.125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGG80.1111111111111111No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG80.1111111111111111No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGG80.1111111111111111No Hit
GGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.1111111111111111No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGG80.1111111111111111No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGG80.1111111111111111No Hit
GGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.1111111111111111No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGG80.1111111111111111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT150.00213594270.037
TGCCGTC150.00213594270.045
CCGTCTT150.00213594270.047
AAGGGGG207.406571E-570.069
AAAAGGG207.406571E-570.067
TCTCGTA150.00213594270.038
ATGCCGT150.00213594270.044
AAAGGGG207.406571E-570.068
GTCTTCT150.00213594270.049
TCGTATG150.00213594270.040
GCCGTCT150.00213594270.046
TCTTCTG150.00213594270.050
AAAAAGG207.406571E-570.066
CGTCTTC150.00213594270.048
CTCGTAT150.00213594270.039
CTTCTGC150.00213594270.051
AAAAAAG252.2345094E-455.99999665
AGCCCAC200.006676138452.517
GTATGCC200.006676138452.542
GCCCACG200.006676138452.518
Produced by FastQC (version 0.11.5)