FastQCFastQC Report
Wed 25 May 2016
SRR1779971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367057
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4123511.233950040456932No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG75752.0637121754931798No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC10720.2920527329542823No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT9430.256908327589448No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG9130.2487352100627423No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTTC150.002223368370.00259422
GTCTCGG200.0069472752.5019442
TAGCGAG305.873074E-446.66839228
GTCGCCG13550.043.9130651
GCCGTAT13350.043.7843954
GATCTCG13600.043.75161741
TCGCCGT13400.043.62101452
AGATCTC13600.043.49425540
CGGTGGT13700.043.43226646
CTCGGTG13750.043.2743344
TCGGTGG13750.043.26843345
TGGTCGC13600.043.23689349
ATCTCGG13800.043.11753542
TCTCGGT13900.042.8073443
TAGATCT13950.042.40339
GTAGATC14000.042.25156438
CGCCGTA13800.042.10300453
CGTATCA14150.041.8036756
CCGTATC14200.041.4099855
GGTGGTC14550.041.13554447