FastQCFastQC Report
Wed 25 May 2016
SRR1779971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367057
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132423.6076140762878786No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT124603.394568146091751No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT122173.3283658941254353No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG22680.617887685018948No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC12800.3487196811394416No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC12330.3359151303476027No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT5130.1397603097066668Illumina PCR Primer Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC4890.13322181568530228No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT4390.11959995314079284No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGG150.002222613770.0085828
GGTACGC150.002223814869.9990515
GCGACGG150.002223814869.9990520
TACGCGA150.002223814869.9990517
GACGACA150.002223814869.9990546
ATTATAC20200.059.256621
AGGGGGG26950.059.09010370
ATCGTAT308.369456E-658.33254239
CGTCTAC553.6379788E-1157.2719548
TCTCCGT252.3890471E-455.99923721
CGTATGA1650.055.15076441
TCGTATG36500.053.98556540
GCCGTCT29600.053.79994246
GTCTTCT23850.053.5632149
TCTCGTA37200.053.44013238
CTCGTAT36900.053.4003639
TATGCCG37500.053.3859443
CGTATGC37250.053.36839741
TCTTCTG24100.053.2980350
CGTCTTC27100.053.20960648