FastQCFastQC Report
Wed 25 May 2016
SRR1779970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543253
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG386837.120623355968582No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG16210.298387675723834No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT150.002224375369.9991766
TCTCGCT200.0069478752.5042117
CGGTCGA200.006950399452.4993827
TCGCCGT2900.045.86152652
CTCGGTG3100.042.90271844
CGTATCA3050.042.45851556
GCCGTAT3050.042.45851554
TGGTCGC3050.042.45851549
TCGGTGG3250.041.99950445
GGACCTA350.001255257439.9995271
GGATTCG350.001255257439.99952762
AGATCTC3300.039.2419640
CGGTGGT3400.039.11718446
CGCCGTA3400.039.11718453
GGGTCTA459.1961316E-538.888431
GTCGCCG3350.038.6562651
GATCTCG3350.038.6562641
ATCTCGG3550.036.47844342
TCTCGGT3800.034.99958843
CAGTTAC400.002418454734.9995841
Produced by FastQC (version 0.11.5)