FastQCFastQC Report
Wed 25 May 2016
SRR1779970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543253
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT257374.737571628688659No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29960.5514925826456549No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG26080.4800709798197157No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC25900.4767576064927391No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT8820.1623552930218517Illumina PCR Primer Index 3 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT6610.1216744316184172No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC5840.10750055683079524No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT207.927904E-569.9945739
CGTATGA352.9746298E-759.99534641
AGGGGGG33500.058.71726670
GTATGAC308.378021E-658.32880442
GACCTAT452.6977432E-854.450241
GCCGTCT36800.052.97143646
CCGTCTT36450.052.80797647
CGTCTTC36500.052.7404948
TCGTATG37750.052.6581740
CGTATGC37600.052.49592241
TATGCCG37300.052.44901743
CTCGTAT37800.052.31075339
TCTCGTA38550.051.7469638
ATGCCGT37900.051.7110344
CTTGAAA38050.051.6958957
TGCCGTC37850.051.68687445
TCTTCTG37050.051.5796950
GTATGCC38000.051.57494442
ATCTCGT38750.051.57019837
AAGGGGG38050.051.51191769