FastQCFastQC Report
Wed 25 May 2016
SRR1779969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555502
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333626.005738953235092No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG10660.1918984990153051No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT200.006948176352.5037814
GACGTAG901.05501385E-1038.8881876
GCCGTAT1900.038.68351754
TGGTCGC2050.037.560349
GTCGCCG2100.036.66600451
TCGCCGT2050.035.85301252
GCGAACT708.684274E-735.0025228
CGAGCAT806.237315E-835.0025218
TATACCG806.242226E-834.999376
ACGAGCA851.0626536E-732.9435517
TACATGC751.4902089E-632.6660772
CGGTGGT2400.032.08275646
CTCGGTG2300.031.95594644
GGTCGGA552.991795E-431.8176125
ACGTAGA1052.0228981E-829.999467
CGTATCA2500.029.39947156
TCGGTGG2500.029.39947145
CGCAAAG1202.2973836E-929.16614212
AGATCTC2550.028.82301140
CGAGGCA500.007204678428.00201829
Produced by FastQC (version 0.11.5)