FastQCFastQC Report
Wed 25 May 2016
SRR1779969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555502
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT215383.8772137634067922No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG24000.4320416488149458No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20070.3612948288214984No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC17930.32277111513549905No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT8520.15337478532930576RNA PCR Primer, Index 34 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT5780.10405003042293277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG32300.054.93772570
GCCGTCT34200.052.8019246
CCGTCTT34100.052.75150747
CGTCTTC34150.052.6790248
TATGCCG35150.052.66917843
CGTATGC35400.052.593841
TCGTATG35750.052.56835640
CGTTCTT200.006947857352.5043919
GGTACGT200.006950331452.4996645
CTCGTAT35250.052.32119439
ATGCCGT35650.051.93047344
GTATGCC35700.051.66168642
TGCCGTC35700.051.66168645
TCTCGTA35900.051.27639438
GTCTTCT34600.051.18464349
ATCTCGT36050.050.96595437
TCTTCTG34900.050.64437550
GGATCTC36750.050.4713235
GATCTCG37050.050.25156436
CTTGAAA35950.050.23611557
Produced by FastQC (version 0.11.5)