FastQCFastQC Report
Wed 25 May 2016
SRR1779968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648935
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245421.4883546046387517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT2400.024.79221352
GCCGTAT2500.023.79980554
CGTATCA2600.022.88512256
GTAGATC6050.021.4060870
ATTGGCG1205.7199875E-520.41649837
ACGGGTC900.005279919619.4431068
GGTCATA3500.018.9986921
GGTCCTA4250.018.939871
CACGCTT950.007213505518.42034140
TACATCG2104.2788088E-818.3320715
TCTCGGT4100.017.92613843
TGGTCGC3153.6379788E-1217.7781749
GTCGCCG3400.017.50038751
CGCCGTA3400.017.50038753
CGGTGGT3850.017.2731146
GACGAAG112400.017.12723270
ACAGCGT1653.3991448E-516.9685298
TCGGTGG3750.016.79986245
GTATAAG7100.016.7594071
GGTTCTA3359.094947E-1216.7152651
Produced by FastQC (version 0.11.5)