FastQCFastQC Report
Wed 25 May 2016
SRR1779968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648935
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT140250.850548990712187No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG17300.10491620348891861No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC17080.10358200899368379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23200.046.76331343
TCGTATG24100.046.6143440
CGTATGC23800.046.17259241
ATGCCGT23750.045.68037444
GCCGTCT23650.045.4295946
CTCGTAT24350.045.41712639
CGTCTTC23800.044.99758548
CCGTCTT23650.044.8363147
TGCCGTC24550.043.906745
GTATGCC24900.043.57063342
AGGGGGG25750.042.81453370
TCTCGTA26050.042.31889738
ATCTCGT26500.041.46821237
GTCTTCT26950.039.8679949
AAGGGGG27650.039.61932469
AATCTCG28300.039.32531436
CTTGAAA28700.038.2917957
CTGCTTG28150.038.0452954
TGCTTGA30900.035.11244655
AAAGGGG31200.035.1113668