FastQCFastQC Report
Wed 25 May 2016
SRR1779967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49555
Sequences flagged as poor quality0
Sequence length76
%GC86

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3418868.99001109877914No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG14162.857431137120371No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC1540.31076581576026635No Hit
TCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGT1520.3067298960750681No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGG930.1876702653617193No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACCAG150.002204355870.0636433
TGCCGGC150.00221321369.99293519
GCAGCAC150.00221321369.9929353
GCGCCGG150.00221321369.99293520
GTGCGCG207.858028E-569.9929354
CCGGCGG150.00221321369.99293521
AAATTTA200.00691579852.494736
TGCGCCG200.00691579852.494719
AAAAAGG1100.050.90395470
GTATCAT2350.050.63318657
CCGTATC2350.050.63318655
CGTATCA2350.050.63318656
TATCATT2350.050.63318658
TCGCCGT2300.050.21232652
TCTCGGT2400.049.57832743
ATCATTA2400.049.57832759
GCCGTAT2400.049.57832754
TCATTAA2400.049.57832760
GTCGCCG2350.049.1439851
CGCCGTA2350.049.1439853
Produced by FastQC (version 0.11.5)