FastQCFastQC Report
Wed 25 May 2016
SRR1779967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49555
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2271245.83190394511149No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG26105.266875189183735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23684.778528907274746No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC17363.5031782867520933No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT5411.0917162748461307No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT5301.06951871657754RNA PCR Primer, Index 14 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC4920.9928362425587731No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT3610.7284835031782868No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC2440.4923822015941883No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT2000.4035919685198264No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT1690.34103521339925336No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT1680.33901725355665424No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1440.2905862173342751No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1350.2724245787508829No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1220.24619110079709414No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT1200.24215518111189585No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT1000.2017959842599132No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC990.19977802441731413No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG970.19574210473211584No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG840.16950862677832712No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT750.15134698819493492No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT750.15134698819493492No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT720.14529310866713754No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT650.1311673897689436No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT610.12309555039854707No Hit
TCTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT560.1130057511855514No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTC207.828814E-570.04545628
CGGTTAA207.828814E-570.04545629
GGTTAAC150.00220662670.0454530
TCCGAGA150.00220662670.0454513
GTTAACA150.00220662670.0454531
ACCATCT252.8070535E-669.9747834
TAAGCCG252.8070535E-669.9747843
TCGAATG252.8070535E-669.9747840
TCTCGAA252.8070535E-669.9747838
AGCATGA252.8070535E-669.9747855
CATATCG252.8070535E-669.9747836
CAGCATG207.868136E-569.9747754
ATCTCGA301.0067015E-769.9747737
CTTTACA301.0067015E-769.974771
TATCGTA207.868136E-569.9747738
CCCATAT207.868136E-569.9747734
TCAGCAT207.868136E-569.9747753
CCATATC207.868136E-569.9747735
ATCGTAT207.868136E-569.9747739
TTTACAC301.0067015E-769.974772
Produced by FastQC (version 0.11.5)