##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779967_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 49555 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.8274846130562 32.0 32.0 32.0 27.0 32.0 2 30.034507113308447 32.0 32.0 32.0 27.0 32.0 3 30.291655736050853 32.0 32.0 32.0 27.0 32.0 4 30.98837655130663 32.0 32.0 32.0 27.0 32.0 5 30.452749470285543 32.0 32.0 32.0 27.0 32.0 6 32.72374129754818 37.0 32.0 37.0 22.0 37.0 7 35.1354051054384 37.0 37.0 37.0 32.0 37.0 8 34.95483805872263 37.0 37.0 37.0 32.0 37.0 9 35.634365856119466 37.0 37.0 37.0 32.0 37.0 10-11 34.93825042881647 37.0 37.0 37.0 29.5 37.0 12-13 35.559136313187366 37.0 37.0 37.0 32.0 37.0 14-15 35.442589042478055 37.0 37.0 37.0 32.0 37.0 16-17 33.5487639995964 37.0 32.0 37.0 24.5 37.0 18-19 34.370981737463424 37.0 37.0 37.0 27.0 37.0 20-21 35.494531328826554 37.0 37.0 37.0 32.0 37.0 22-23 34.64952073453738 37.0 37.0 37.0 29.5 37.0 24-25 34.71077590555948 37.0 37.0 37.0 27.0 37.0 26-27 34.410745636161835 37.0 34.5 37.0 27.0 37.0 28-29 34.82954293209565 37.0 37.0 37.0 29.5 37.0 30-31 34.88635859146403 37.0 37.0 37.0 32.0 37.0 32-33 34.93623246897387 37.0 37.0 37.0 32.0 37.0 34-35 33.56549288669156 37.0 34.5 37.0 24.5 37.0 36-37 32.09763898698416 37.0 32.0 37.0 17.5 37.0 38-39 31.86998284734134 37.0 29.5 37.0 13.0 37.0 40-41 33.22163252951266 37.0 34.5 37.0 20.0 37.0 42-43 34.093007769145395 37.0 37.0 37.0 24.5 37.0 44-45 34.51463020885885 37.0 37.0 37.0 27.0 37.0 46-47 33.82164261931187 37.0 34.5 37.0 24.5 37.0 48-49 32.6889012208657 37.0 32.0 37.0 17.5 37.0 50-51 33.33508223186359 37.0 34.5 37.0 22.0 37.0 52-53 32.377862980526686 37.0 29.5 37.0 17.5 37.0 54-55 32.50505498940571 37.0 32.0 37.0 17.5 37.0 56-57 32.55348602562809 37.0 32.0 37.0 17.5 37.0 58-59 32.52937140550903 37.0 29.5 37.0 17.5 37.0 60-61 33.34172131974574 37.0 34.5 37.0 20.0 37.0 62-63 34.501251135102414 37.0 37.0 37.0 27.0 37.0 64-65 34.60303702956311 37.0 37.0 37.0 27.0 37.0 66-67 34.350670971647666 37.0 37.0 37.0 27.0 37.0 68-69 33.6222782766623 37.0 34.5 37.0 22.0 37.0 70-71 30.64678639895066 37.0 27.0 37.0 13.0 37.0 72-73 33.16766219352235 37.0 34.5 37.0 17.5 37.0 74-75 34.419412773685806 37.0 37.0 37.0 27.0 37.0 76 34.69684189284633 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -0.7777270046133324 11101 2 -0.3963647352584623 11101 3 1.5310580698768632 11101 4 0.8052079251426321 11101 5 0.020854034640688468 11101 6 -0.598955382995694 11101 7 -0.9497759522618807 11101 8 0.48348342395907906 11101 9 0.4771913626155495 11101 10-11 -1.6171937243377954 11101 12-13 -0.6440107337673098 11101 14-15 -1.1045637750969206 11101 16-17 -0.9125485838258456 11101 18-19 0.6567753366067066 11101 20-21 0.4091542414967506 11101 22-23 0.33544560688584113 11101 24-25 0.035899225115251454 11101 26-27 -1.137338389360366 11101 28-29 0.5828482370030557 11101 30-31 -0.0431413411229471 11101 32-33 -1.0581916096916473 11101 34-35 1.714103322983199 11101 36-37 -0.4488467167823842 11101 38-39 0.3423322283358985 11101 40-41 0.18940227781288854 11101 42-43 0.7735730956826075 11101 44-45 0.12880585454213644 11101 46-47 -0.276333839699781 11101 48-49 -0.22659593543973244 11101 50-51 0.5742268137570079 11101 52-53 -1.9762567027706623 11101 54-55 -1.3802031940926582 11101 56-57 -1.8533381498790682 11101 58-59 -0.7161368788206346 11101 60-61 0.7692686315090569 11101 62-63 -1.7903655385630302 11101 64-65 0.5417721348993325 11101 66-67 0.5381981456036584 11101 68-69 -0.34251330093017174 11101 70-71 -0.8554153548522301 11101 72-73 -1.3395941186509148 11101 74-75 -0.5948486372629063 11101 76 1.1316613795240684 11102 1 0.2179439910576626 11102 2 0.3936785547848274 11102 3 -3.0511930123742204 11102 4 -0.11037649044178366 11102 5 -0.7670247532380969 11102 6 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26-27 -0.17561590132208948 21612 28-29 -0.4410752079730287 21612 30-31 -0.13165808753443287 21612 32-33 -1.0713495044284898 21612 34-35 0.5526200693946777 21612 36-37 0.16239730235637495 21612 38-39 0.3208011278574361 21612 40-41 1.3197850529325095 21612 42-43 0.4529989330031725 21612 44-45 0.8213895865995511 21612 46-47 1.3325178349413704 21612 48-49 1.4899112415937594 21612 50-51 0.08738470849385038 21612 52-53 0.00580071349727973 21612 54-55 1.2370216862901202 21612 56-57 -0.4705630302618431 21612 58-59 -1.2424526682943196 21612 60-61 0.8063499712219837 21612 62-63 0.48475407866185094 21612 64-65 0.3013415128897634 21612 66-67 0.6255187197663332 21612 68-69 -0.25519272676749694 21612 70-71 0.46755115232480193 21612 72-73 -1.4376802430528315 21612 74-75 0.6228547120193895 21612 76 -0.942501299901771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 10.0 20 22.0 21 61.0 22 145.0 23 214.0 24 278.0 25 397.0 26 632.0 27 851.0 28 1108.0 29 1336.0 30 1751.0 31 2581.0 32 3972.0 33 6685.0 34 11083.0 35 14054.0 36 4373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.17847196997215 6.8490939177462975 11.119183113371271 67.85325099891027 2 9.6867936712948 9.055134000645786 66.74806264126573 14.510009686793671 3 10.194938854582878 10.681276990757556 68.50708318198329 10.616700972676272 4 8.297850872767631 60.9242256079104 21.69306830794067 9.084855211381292 5 8.86086166885279 18.30491373221673 68.04964181212793 4.784582786802543 6 12.019211365379183 69.45352544698713 11.494531218468742 7.032731969164951 7 11.498335183129855 16.72485117546161 10.525678538997074 61.25113510241146 8 10.721420643729191 70.11603269094945 5.989304812834225 13.173241852487136 9 9.92836242558773 13.106649177681366 5.046917566340429 71.91807083039048 10-11 11.069630406925707 40.402990646661756 34.44994904599986 14.077429900412678 12-13 11.474119665018666 8.453233780647764 40.60740591262234 39.46524064171123 14-15 12.16426193118757 6.366663303400262 11.41963474926849 70.04944001614368 16-17 43.256987185955 34.11159317929573 5.0378367470487335 17.593582887700535 18-19 45.54379521536894 13.205662452451342 5.216478826342714 36.034063505837004 20-21 14.451619412773686 6.889314902633438 5.04590858641913 73.61315709817374 22-23 11.957421047321159 38.44818888104127 5.012612249016245 44.58177782262133 24-25 43.42333615853413 38.0504096541147 5.176171449328006 13.350082738023167 26-27 46.346902940642224 40.68561164147174 5.2868993077280155 7.680586110158032 28-29 15.499581201497584 12.639641548848052 5.963085182606238 65.89769206704813 30-31 70.73146120702704 5.649677608145061 7.1602272383278 16.458633946500107 32-33 20.232088799192734 6.125126135216952 65.40060544904136 8.24217961654894 34-35 10.369809797689319 7.616164673830786 16.183845416477475 65.83018011200242 36-37 10.100897992129957 34.57875088285743 7.266673393199476 48.05367773181314 38-39 10.915144788618706 12.782766622944203 37.9104025829886 38.39168600544849 40-41 10.889131048956694 6.599870847963837 41.290309561286676 41.220688541792796 42-43 42.233724825442955 6.222504742301328 38.39548775073657 13.148282681519149 44-45 13.694884471799012 36.9871859549995 41.73342750479265 7.584502068408838 46-47 41.80935756303793 8.77940004237844 14.429858637634071 34.981383756949555 48-49 40.78700433861366 5.778428009282615 8.198970840480275 45.235596811623445 50-51 15.987286852991625 6.135606901422662 39.57824639289678 38.298859852688935 52-53 9.452123902734336 6.939763898698416 69.70537786298053 13.902734335586722 54-55 9.78912319644839 6.994248814448592 44.86933710019171 38.34729088891131 56-57 41.02209666027646 6.068005246695591 14.60195742104732 38.30794067198063 58-59 12.95429320956513 8.586419130259308 64.8763999596408 13.58288770053476 60-61 40.58924427403895 36.71274341640601 15.48784179194834 7.2101705176066995 62-63 12.434794014670716 74.90439818785379 5.461663421081839 7.199144376393668 64-65 9.315184598464286 78.46569868929541 4.933052155750855 7.286064556489451 66-67 9.406185358962716 78.45719186721153 4.623379244235911 7.513243529589829 68-69 11.166380789022298 76.89435980223993 4.692765613964283 7.246493794773484 70-71 38.85682574916759 49.29169609524771 4.428412874583796 7.423065281000908 72-73 79.75037333010452 8.463494369778424 4.388142228679824 7.39799007143722 74-75 83.10160427807487 4.911714256886288 4.726061951367168 7.260619513671678 76 83.24622121768611 4.609206304360987 4.988598066716445 7.155974411236454 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 5.5 17 10.0 18 11.5 19 23.0 20 24.0 21 15.0 22 28.0 23 43.5 24 70.0 25 94.0 26 90.0 27 82.0 28 82.5 29 87.0 30 109.5 31 139.0 32 159.5 33 173.0 34 175.0 35 189.5 36 257.0 37 312.0 38 315.5 39 312.5 40 343.0 41 376.5 42 373.0 43 409.5 44 477.5 45 512.0 46 515.0 47 695.0 48 1541.5 49 5169.5 50 8131.0 51 16144.0 52 12735.5 53 1175.5 54 1037.0 55 745.0 56 513.0 57 517.0 58 461.0 59 465.5 60 456.5 61 436.0 62 429.0 63 414.0 64 358.0 65 300.0 66 259.0 67 235.0 68 210.5 69 177.0 70 133.0 71 98.0 72 96.5 73 86.5 74 72.0 75 66.0 76 66.5 77 45.5 78 24.0 79 24.0 80 19.0 81 10.5 82 8.0 83 9.0 84 6.5 85 5.0 86 6.0 87 6.0 88 3.5 89 2.0 90 2.0 91 1.0 92 1.0 93 2.0 94 3.0 95 3.0 96 3.0 97 9.0 98 43.0 99 1196.5 100 2322.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002017959842599132 2 0.006053879527797397 3 0.002017959842599132 4 0.0 5 0.0 6 0.002017959842599132 7 0.0 8 0.0 9 0.0 10-11 0.001008979921299566 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.001008979921299566 20-21 0.0 22-23 0.0 24-25 0.002017959842599132 26-27 0.016143678740793057 28-29 0.01715265866209262 30-31 0.007062859449096962 32-33 0.010089799212995663 34-35 0.005044899606497831 36-37 0.0 38-39 0.0 40-41 0.002017959842599132 42-43 0.002017959842599132 44-45 0.0 46-47 0.0030269397638986984 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.001008979921299566 64-65 0.0030269397638986984 66-67 0.005044899606497831 68-69 0.0 70-71 0.0 72-73 0.002017959842599132 74-75 0.0 76 0.004035919685198264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 49555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.203107658157602 #Duplication Level Percentage of deduplicated Percentage of total 1 68.17595307917888 11.728382605186157 2 11.964809384164223 4.1166380789022305 3 8.0 4.128745837957824 4 4.762463343108505 3.2771667843809906 5 2.8269794721407626 2.4316416103319543 6 1.5835777126099706 1.634547472505297 7 0.8797653958944283 1.0594289173645446 8 0.375366568914956 0.5165977197053778 9 0.16422287390029325 0.2542629401674907 >10 0.9618768328445748 3.3800827363535464 >50 0.11730205278592376 1.5820805165977196 >100 0.11730205278592376 4.34870346080113 >500 0.02346041055718475 2.1612349914236706 >1k 0.03519061583577712 13.548582383210576 >5k 0.0 0.0 >10k+ 0.011730205278592375 45.83190394511149 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 22712 45.83190394511149 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG 2610 5.266875189183735 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 2368 4.778528907274746 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC 1736 3.5031782867520933 No Hit CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 541 1.0917162748461307 No Hit ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT 530 1.06951871657754 RNA PCR Primer, Index 14 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC 492 0.9928362425587731 No Hit CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 361 0.7284835031782868 No Hit CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC 244 0.4923822015941883 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT 200 0.4035919685198264 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT 169 0.34103521339925336 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT 168 0.33901725355665424 No Hit CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC 144 0.2905862173342751 No Hit CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG 135 0.2724245787508829 No Hit TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC 122 0.24619110079709414 No Hit TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT 120 0.24215518111189585 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT 100 0.2017959842599132 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC 99 0.19977802441731413 No Hit CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG 97 0.19574210473211584 No Hit CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG 84 0.16950862677832712 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT 75 0.15134698819493492 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT 75 0.15134698819493492 No Hit CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 72 0.14529310866713754 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT 65 0.1311673897689436 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT 61 0.12309555039854707 No Hit TCTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT 56 0.1130057511855514 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010089799212995661 0.0 2 0.0 0.0 0.0 0.010089799212995661 0.0 3 0.0 0.0 0.0 0.012107759055594794 0.0 4 0.0 0.0 0.0 0.012107759055594794 0.0 5 0.0 0.0 0.0 0.016143678740793057 0.0 6 0.0 0.0 0.0 0.016143678740793057 0.0 7 0.0 0.0 0.0 0.016143678740793057 0.0 8 0.0 0.0 0.0 0.016143678740793057 0.0 9 0.0 0.0 0.0 0.016143678740793057 0.0 10 0.0 0.0 0.0 0.01816163858339219 0.0 11 0.0 0.0 0.0 0.01816163858339219 0.0 12 0.0 0.0 0.0 0.01816163858339219 0.0 13 0.0 0.0 0.0 0.02623347795378872 0.0 14 0.0 0.0 0.0 0.02623347795378872 0.0 15 0.0 0.0 0.0 0.030269397638986985 0.0 16 0.0 0.0 0.0 0.030269397638986985 0.0 17 0.0 0.0 0.0 0.030269397638986985 0.0 18 0.0 0.0 0.0 0.03632327716678438 0.0 19 0.0 0.0 0.0 0.048431036222379174 0.0 20 0.0 0.0 0.0 0.048431036222379174 0.0 21 0.0 0.0 0.0 0.05448491575017657 0.0 22 0.0 0.0 0.0 0.0625567551205731 0.0 23 0.0 0.0 0.0 0.08273635354656442 0.0 24 0.0 0.0 0.0 0.08879023307436182 0.0 25 0.0 0.0 0.0 0.11300575118555141 0.0 26 0.0 0.0 0.0 0.12309555039854707 0.0 27 0.0 0.0 0.0 0.16950862677832712 0.0 28 0.0 0.0 0.0 0.22802946221370196 0.0 29 0.0 0.0 0.0 0.274442538593482 0.0 30 0.0 0.0 0.0 0.31681969528806375 0.0 31 0.0 0.0 0.0 0.34507113308445164 0.0 32 0.0 0.0 0.0 0.3854303299364343 0.0 33 0.0 0.0 0.0 0.5065079204923822 0.0 34 0.0 0.0 0.0 0.6033699929371406 0.0 35 0.0 0.0 0.0 0.7950761779840582 0.0 36 0.0 0.0 0.0 1.0352133992533548 0.0 37 0.0 0.0 0.0 1.4287155685601856 0.0 38 0.0 0.0 0.0 1.7334275047926546 0.0 39 0.0 0.0 0.0 2.2459893048128343 0.0 40 0.0 0.0 0.0 2.6475633134900614 0.0 41 0.0 0.0 0.0 3.0874785591766725 0.0 42 0.0 0.0 0.0 3.482998688326102 0.0 43 0.0 0.0 0.0 3.9915245686610836 0.0 44 0.0 0.0 0.0 4.522248007264656 0.0 45 0.0 0.0 0.0 4.97628897184946 0.0 46 0.0 0.0 0.0 5.365755221471093 0.0 47 0.0 0.0 0.0 5.757239430935324 0.0 48 0.0 0.0 0.0 6.144687720714358 0.0 49 0.0 0.0 0.0 6.5159923317525985 0.0 50 0.0 0.0 0.0 6.905458581374231 0.0 51 0.0 0.0 0.0 7.196044798708506 0.0 52 0.0 0.0 0.0 7.490666935727979 0.0 53 0.0 0.0 0.0 7.819594390071638 0.0 54 0.0 0.0 0.0 8.071839370396528 0.0 55 0.0 0.0 0.0 8.318030471193623 0.0 56 0.0 0.0 0.0 8.62879628695389 0.0 57 0.0 0.0 0.0 8.850771869639795 0.0 58 0.0 0.0 0.0 9.125214408233276 0.0 59 0.0 0.0 0.0 9.312884673594995 0.0 60 0.0 0.0 0.0 9.595399051558873 0.0 61 0.0 0.0 0.0 9.843608112198567 0.0 62 0.0 0.0 0.0 10.107960851579053 0.0 63 0.0 0.0 0.0 10.2350923216628 0.0 64 0.0 0.0 0.0 10.378367470487337 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGGTTC 20 7.828814E-5 70.045456 28 CGGTTAA 20 7.828814E-5 70.045456 29 GGTTAAC 15 0.002206626 70.04545 30 TCCGAGA 15 0.002206626 70.04545 13 GTTAACA 15 0.002206626 70.04545 31 ACCATCT 25 2.8070535E-6 69.97478 34 TAAGCCG 25 2.8070535E-6 69.97478 43 TCGAATG 25 2.8070535E-6 69.97478 40 TCTCGAA 25 2.8070535E-6 69.97478 38 AGCATGA 25 2.8070535E-6 69.97478 55 CATATCG 25 2.8070535E-6 69.97478 36 CAGCATG 20 7.868136E-5 69.97477 54 ATCTCGA 30 1.0067015E-7 69.97477 37 CTTTACA 30 1.0067015E-7 69.97477 1 TATCGTA 20 7.868136E-5 69.97477 38 CCCATAT 20 7.868136E-5 69.97477 34 TCAGCAT 20 7.868136E-5 69.97477 53 CCATATC 20 7.868136E-5 69.97477 35 ATCGTAT 20 7.868136E-5 69.97477 39 TTTACAC 30 1.0067015E-7 69.97477 2 >>END_MODULE