FastQCFastQC Report
Wed 25 May 2016
SRR1779966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243535
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3741615.36370542221857No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG13110.5383209805572094No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTCG150.002222974369.99856662
CTGGCGT150.002222974369.9985665
TGTGCGC252.3875259E-455.9988483
CGCGTGT252.3875259E-455.9988487
CGCGTCT252.3875259E-455.9988487
TACGGAC200.006946054852.4989248
TGTTCGA200.006946054852.49892463
CCGAGTG200.006946054852.49892443
GCATGCA200.006946054852.49892448
GGCTACC200.006946054852.49892457
CTAGCTA200.006946054852.49892412
CCCGCTT305.870823E-446.6657074
GCGTGTG404.584647E-543.7491048
TGCGCGT503.470048E-641.9991345
GCGGTTT350.001252681440.00739316
GCGCGTC350.001253947439.999186
GTTACTC350.001253947439.999189
GGTATAC350.001253947439.999181
TGGCGCT753.5232006E-837.33256513
TTTGCGG400.002415946234.99928313
Produced by FastQC (version 0.11.5)