FastQCFastQC Report
Wed 25 May 2016
SRR1779966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243535
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT142875.866507894142526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23770.9760404048699366No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT21970.9021290574250107No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG10050.41267168990083564No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC8880.36462931406163385No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT7750.31822941261009713No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT4970.2040774426673784No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT4080.16753238754183178No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT3590.14741207629293532No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG3140.12893423943170387No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT2960.12154310468721129No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT2920.11990063029954626No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGC150.002215944770.0544722
TTCGCGT150.002217752270.0400729
CCGGATT252.8384711E-669.98254447
CCGTGAT252.8384711E-669.98254447
GTCCGGA150.00222499369.9825442
TAAGACG252.8384711E-669.98254443
AGGGGGG23800.061.4678870
ATGCCGG650.059.21644
TACGCGG308.3320265E-658.36672629
GACGTAT308.372723E-658.31878746
CTCGGAA308.372723E-658.31878739
CTGCTTG5850.058.03163554
ATTAAGC1450.057.92848651
CCGTCTT11400.057.70490347
CGTATGC20500.057.6929341
GCCGTCT11000.057.5765546
TATGCCG24150.057.5218943
GCCGTAT1950.057.42157746
TCGTATG21000.057.3190440
CGTCTTC7350.057.14034348
Produced by FastQC (version 0.11.5)