FastQCFastQC Report
Wed 25 May 2016
SRR1779965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335626
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG330079.834458593791899No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG12410.3697568126426439No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT150.002223922269.996876
CTTGATT200.00694490552.50547820
CGTTCTA200.00694899952.4976510
ATATACG200.00694899952.497655
CTGGTAT305.874716E-446.6645836
GCCGTAT2900.042.2394954
TCGGTGG3100.040.64334545
TCGTTTA350.001253855540.00417320
CGAATAT350.001254774639.9982130
CGTTTAT350.001254774639.9982121
TCGCCGT3200.039.3791152
TAGCGCC459.174276E-538.89874315
TGGTCGC3350.038.66074849
CTCGGTG3200.038.27953744
TCTCGGT3400.038.08653343
CGGTGGT3400.038.08653346
ATCTCGG3350.036.5655342
CGTATCA3350.036.5655356
GATCTCG3400.036.027841
CGCCGTA3500.036.00375453
Produced by FastQC (version 0.11.5)