FastQCFastQC Report
Wed 25 May 2016
SRR1779965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335626
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT210016.257262548193525No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG23080.6876702043345867No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22810.6796255355663745No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC20440.6090112208231782No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT7390.22018556369292008Illumina PCR Primer Index 11 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT6460.19247614904685573No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT5810.17310935386412254No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC3870.11530691901104205No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3720.11083765858425748No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT150.002223584669.9995566
GGACCTA150.002224899169.989121
GTGCGGA150.002224899169.9891211
AGGGGGG28350.060.61689870
TGAGTCG252.3886653E-455.9996466
ATCGTAT252.3904248E-455.99129539
ATATCGT252.3904248E-455.99129537
CCGACTT252.3904248E-455.99129547
AACGTAT200.00694385152.5074964
ACGTATA200.00694385152.5074965
CCATTGA200.00694794552.49966415
CCGTCTT33000.051.74953547
CGTCTTC33550.051.43037448
GCCGTCT33300.051.38841246
AAGGGGG33450.051.37485569
TCTTCTG33600.051.3538450
TATGCCG33700.051.29766543
TCGTATG34200.051.1616440
CGTATGC34000.051.15381641
GTCTTCT33950.051.030649