FastQCFastQC Report
Wed 25 May 2016
SRR1779964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175169
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2718015.51644411967871No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG11240.641666048216294No Hit
ATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTGGTC2110.1204551033573292No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCCG150.002221388370.0028523
GAATGTC150.002223905969.98286458
CGAGAAT150.002223905969.98286414
CGGTGGT1750.054.00220546
GATCTCG1850.052.97513641
CCGAAAA200.006933290552.5171427
AAGTCGT200.006941128552.50214419
TTCGTAC200.00694897352.4871563
GCTTACC200.00694897352.48715660
TCGTACA200.00694897352.4871564
ATGCGGA200.00694897352.48715654
TCGCCGT1850.051.08316852
CTCGGTG1850.051.08316844
ATCTCGG2000.050.75206842
GCCGTAT1800.050.54318654
AGATCTC1900.049.73887340
TGGTCGC1900.049.73887349
TCTCGGT1950.048.4635243
CGCCGTA1950.048.4635253
TCGGTGG1950.048.4635245
Produced by FastQC (version 0.11.5)