FastQCFastQC Report
Wed 25 May 2016
SRR1779964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175169
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT171739.803675307845566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22111.2622096375500231No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC18821.074391016675325No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG17480.9978934628844145No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT6510.37164110087972185TruSeq Adapter, Index 9 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT6020.3436681147920009No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3840.21921687056499725No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC3540.20209055255210684No Hit
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC2390.13643966683602693No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG150.0022204970.0099930
ACAATAC150.002223006769.9900053
CGTAATC150.002223006769.99000548
AGGGGGG23300.061.42970
CGTCTAC308.357665E-658.3250148
ATGACGT700.054.99215344
AAGGGGG26550.053.77763469
TCGGAGT200.006930497552.5224965
TGCGATT200.00693833252.50749217
TTGCGAT200.00693833252.50749216
AATGGAC200.006946173552.49250434
TATGACG800.052.49250443
ATATCGT407.4549826E-752.49250437
GCTTATC200.006946173552.49250411
AACTCCG407.4549826E-752.49250410
ACTCCGA407.4549826E-752.49250411
TATCGTA407.4549826E-752.49250438
AGATCTC27700.051.7976635
ATCTCGT28100.051.55847537
GATCTCG28100.051.4339436