FastQCFastQC Report
Wed 25 May 2016
SRR1779963_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779963_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217939
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3370115.463501254938308No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG16140.7405741973671531No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG150.002221640470.0066520
GGATCTA200.006948207552.49294712
GGTAGCG200.006948207552.4929477
TATGGAT305.8727514E-446.66039746
CGCCGTA3200.042.6603153
TCATTAA3000.042.00399460
CCGTATC3050.041.31540355
GCCGTAT3100.040.64902554
TCGCCGT3050.040.1585452
CGTATCA3200.039.37874656
GTGGTCG3300.039.23715248
ATCATTA3150.038.89258659
TGGTCGC3350.038.6515249
CGGTGGT3550.037.45975546
GTATCAT3400.037.06234757
GGTGGTC3500.036.99502647
CATTAAA3550.036.4823461
CTCGGTG3550.036.4739744
GGTCGCC3550.035.48818650
TCTCGGT3650.035.48282643
Produced by FastQC (version 0.11.5)