FastQCFastQC Report
Wed 25 May 2016
SRR1779963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217939
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2231110.23726822643033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27721.271915535998605No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG23091.0594707693437155No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC21240.9745846314794507No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC4770.2188685824932665No Hit
CATATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT4530.2078563267703348No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT4420.20280904289732446TruSeq Adapter, Index 2 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT2600.11929943699842617No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT2350.10782833728703904No Hit
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC2340.10736949329858356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTA600.070.00619545
TTTGCGA150.002223721769.9901354
AGGGGGG27300.065.26218470
GCCGTAT553.6379788E-1157.27779846
CCGTCTT29500.056.47957247
CTGCTTG27300.056.40230654
CGTCTTC29400.055.94449648
GCCGTCT29850.055.93459746
TGCTTGA28300.055.8931855
GTCTTCT28200.055.8431949
TCTGCTT27650.055.68835453
TATGCCG32950.055.55878443
ATGCCGT32950.055.45255344
AAGGGGG32050.055.37151769
CTTGAAA30450.055.2795657
TCGTATG33200.055.24585340
TTCTGCT26800.055.2347552
TGCCGTC32400.055.205545
CGTATGC32550.055.05863641
GCTTGAA30650.054.690556