FastQCFastQC Report
Wed 25 May 2016
SRR1779962_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779962_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252521
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2728810.80622997691281No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG11020.4363993489650365No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTC150.00222288970.056
ACTGTAC207.91666E-570.08
ATTCGGT305.870644E-446.66666418
AATTCGG305.870644E-446.66666417
GCCGTAT1850.043.51351554
CCGCCTA659.9717E-943.07692315
TGGTCGC2000.042.049
GATCTCG2050.040.9756141
ATCTCGG2100.040.042
ACGTTCT350.001253909140.039
CAATTCG350.001253909140.016
CGTTCTC350.001253909140.040
CGGTGGT2100.040.046
CTCGGTG2050.039.2682944
TCGCCGT2100.038.33333252
TCGGTGG2100.038.33333245
GTCGCCG2200.038.18181651
TCTCGGT2400.037.91666443
CCATAGC654.850044E-737.6923079
AGATCTC2150.037.4418640
Produced by FastQC (version 0.11.5)