FastQCFastQC Report
Wed 25 May 2016
SRR1779962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252521
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT178237.058026857172275No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC21330.8446822244486597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19970.7908253174983467No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG18200.7207321371291893No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT6590.2609683947077669No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4740.18770716098859105RNA PCR Primer, Index 19 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC3780.14969052078837006No Hit
CTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT3010.11919800729444283RNA PCR Primer, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG21250.061.5947770
CGCGTAA352.964298E-760.0067928
AAGCCGT352.9683542E-759.99490744
ACGCGTA452.6899215E-854.45060727
TATGCCG25000.053.89542443
CGTATGC25400.053.8733841
GCCGTCT24700.053.84158346
CCGTCTT24650.053.52486847
CGTCTTC24750.053.4500148
TCTTCTG24850.053.3757550
CTCGTAT25600.053.3157939
TCGTATG25700.053.2445140
GTATGCC25350.053.1513142
TCTCGTA25900.053.103638
GTCTTCT25100.052.8441249
ATGCCGT25300.052.84136644
TGCCGTC25200.052.773345
CTGCTTG25400.052.7711154
CTTGAAA25850.052.66477657
AGCCGTC407.465278E-752.49554445