FastQCFastQC Report
Wed 25 May 2016
SRR1779961_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779961_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349161
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4305212.33012850805216No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG17300.49547343489106743No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCG150.00222351370.0007235
ACACCGT150.002224776769.990696
AATATCG200.00694772352.5005349
GGGACGT200.00694772352.50053442
TCCACGT200.00694772352.50053450
GGCCGGT200.0069516652.493027
CGCGTCT200.0069516652.493027
GCCGTAT3350.045.97061554
TCGGTGG3800.045.13204245
TCTCGGT3750.044.8004643
TTCGTGA551.3089993E-744.54591422
GGTTCAC551.3089993E-744.54591433
TGGTCGC3700.042.56800549
CTCGGTG3950.042.53207844
TCGCCGT3550.042.39480252
AGATCTC3800.042.36885540
CGCCGTA3600.041.80598453
ATCTCGG3900.041.28247542
GATCTCG3900.041.28247541
CGGTGGT4000.041.1254246