FastQCFastQC Report
Wed 25 May 2016
SRR1779961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349161
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT286238.197650940397123No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30910.8852649637273349No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG28710.8222567812556385No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC24780.7097012552948354No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT9550.2735127920930459No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT7670.2196694361626871No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5400.15465644788507307No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC5090.14577802217315222No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT3710.10625470771363353No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3650.10453630273713273No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGT207.92154E-569.99885637
AGGGGGG35950.062.59980870
AAGGGGG42600.052.74561769
CGTATGC44000.052.73777441
TCGTATG44000.052.73777440
CTCGTAT43700.052.61927439
CGTCTTC42950.052.56025748
GCCGTCT42900.052.53993246
CAGACGT200.00694451852.506661
CGTTGCG200.00694845352.49914627
TCGAAAG200.00694845352.49914640
TATGCCG43750.052.47913743
CCGTCTT42650.052.4375947
CTTGAAA44100.052.38009357
TCTCGTA44000.052.18096538
TCTTCTG43400.052.17656750
GTATGCC43950.052.16069442
ATCTCGT43800.052.09960637
GTCTTCT43650.052.03809449
TTGAAAA44850.051.8943658