FastQCFastQC Report
Wed 25 May 2016
SRR1779960_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779960_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285079
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3048610.693877837371394No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG11990.42058517112800314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCCG150.002223227969.9996516
TCGATCC150.002223227969.9996530
ACGACGC150.002223227969.9996521
TACCGAC150.002223227969.9996567
TGTGGAC200.006942021752.50894569
TACTTTC200.006946840352.49973730
AGGACGT200.006946840352.4997376
ACCGACG200.006946840352.49973768
ATCCGCG200.006946840352.49973733
GGACGTA200.006946840352.4997377
AGTTCCG200.006946840352.4997377
TCCCGCT200.006946840352.49973710
GACGCGC200.006946840352.49973723
GCGCTCG200.006946840352.49973726
GCTCGAT200.006946840352.49973728
CGTTTTT200.006946840352.49973742
TACCGGC200.006946840352.4997374
TAGAGCC404.586209E-543.749784
GGAACCC404.586209E-543.7497854
AACGAAG350.001253129940.0068270
Produced by FastQC (version 0.11.5)