FastQCFastQC Report
Wed 25 May 2016
SRR1779960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285079
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT202917.11767615292603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21940.769611230571175No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG20620.7233082759515783No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC19820.6952458792124288No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT5410.1897719579484985TruSeq Adapter, Index 7 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4130.1448721231658593No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC4050.14206588349194435No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT3100.10874178736420431No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT150.002223338269.9987713
CTGACCG207.919097E-569.998779
CGCGTCG150.002223338269.9987724
GCGTCGT150.002223338269.9987725
TACGTCC150.002223338269.9987739
CGCTTGG207.919097E-569.9987714
AGGGGGG24700.062.06368670
CCGCTTG252.3881844E-455.99901613
GATCGAG200.006947184452.4990817
GCGCGTC200.006947184452.4990823
GGATCGA200.006947184452.4990816
TGACGTC800.052.4990845
CACGACT200.006947184452.4990833
AGGCGCA200.006947184452.4990857
TCGTCGT200.006947184452.499086
CAAATCG200.006947184452.4990836
GTCCACG200.006947184452.4990830
CGTCCAC200.006947184452.4990829
GCCGTAA200.006947184452.4990846
ACGACTA200.006947184452.4990834
Produced by FastQC (version 0.11.5)