FastQCFastQC Report
Wed 25 May 2016
SRR1779959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212076
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4204319.824496878477525No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG17740.8364925781323677No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT150.002220525470.0148631
GGTCTAA150.002222603769.998351
CAATACG150.002222603769.9983568
TCACGTA150.002222603769.9983525
CTCGTCG200.00693843252.51114731
GGTAATG200.006944907452.4987641
ATCTAGC200.006944907452.4987643
CGTTGAA200.006944907452.49876442
TCGTCGA200.006944907452.49876423
CAGGACG407.456383E-752.4987644
ATGCGGA200.006944907452.4987647
CGTAACA200.006944907452.49876457
CCGGTCT200.006944907452.4987649
CACGTAG200.006944907452.49876426
ATGTATA305.869184E-446.6655736
TCGCCGT3550.045.35104452
GCCGTAT3550.045.35104454
CGTATCA3650.045.06743256
GTCGCCG3650.044.1085551
CTCGGTG3650.044.1085544
Produced by FastQC (version 0.11.5)