FastQCFastQC Report
Wed 25 May 2016
SRR1779959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212076
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2858913.480544710386845No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31691.4942756370357797No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG29891.4094004036288879No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC23191.093475923725457No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT9290.4380505101944586RNA PCR Primer, Index 19 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT8900.419660876289632No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC6420.3027216658179143No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT5890.2777306248703295No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC3300.15560459457930176No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA150.002220525470.0148667
TAACGGA150.002220525470.0148667
AATATCG207.914662E-569.9983536
AACTCGT207.914662E-569.9983537
TATAAGC150.002222603769.9983533
GCCGTAT252.8334034E-669.99834446
AGGGGGG36250.065.1708870
CGTCTAC401.0617441E-861.24855848
CGAATGC401.0617441E-861.24855841
CGTCTTA252.3868562E-455.99867648
AACGGAG252.3868562E-455.99867668
TGCCGTA252.3868562E-455.99867645
AAGGGGG42100.055.86566569
CGTATGC44650.055.10508341
GCCGTCT43700.055.10167746
TATGCCG44150.055.09496343
TCGTATG45300.055.08699840
CCGTCTT43350.055.06213847
ATGCCGT44200.055.0326444
CGTATGA700.054.99870341