FastQCFastQC Report
Wed 25 May 2016
SRR1779958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80086
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4091751.09132682366456No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG57787.214744150038708No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC6500.8116275004370301No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT6500.8116275004370301No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG6360.7941462927353096No Hit
TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG1620.2022825462627675No Hit
CTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGG1520.18979596933296705No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG860.10738456159628398No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAAAT207.883713E-570.0000119
TGTATAT207.883713E-570.0000137
TTAAGGT150.002217329870.0000149
CGAATCA150.002217329870.0000156
GTATATC207.883713E-570.0000138
CCGAATC150.002217329870.0000155
CTTTACA150.002217329870.000011
TCTCTGT308.309384E-658.33333645
TATATCT252.3775769E-456.00000439
ATTTAAG200.006928550552.50000447
GGAACCA200.006928550552.50000421
TTTATTC200.006928550552.50000453
TAAGGTC200.006928550552.50000450
TCTGGAT200.006928550552.5000041
CAGACGC200.006928550552.50000455
TTTATAC352.0699514E-550.0000043
TCATTAA10450.048.22966860
ATCATTA10450.048.22966859
GTATCAT10400.048.12500457
GCCGTAT10450.047.89473754