FastQCFastQC Report
Wed 25 May 2016
SRR1779958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80086
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT2744634.27065904153036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG980412.241840021976376No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG32874.104337836825413No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC24243.0267462477836324No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT11711.4621781584796343No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT9631.2024573583397848Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC7260.9065254851035136No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC4620.5768798541567814No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT2370.295931873236271No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT2160.26971006168369No Hit
TATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCT2120.26471543091176986No Hit
TTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC2010.2509801962889894No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC2000.24973153859600927No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG1900.23724496166620881No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAATGCATCTCGTATGCCGT1370.17106610393826635No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC1230.1535848962365457No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTACTGCATCTCGTATGCCGT1230.1535848962365457No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG1220.15233623854356565No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGAATCTCGTATGCCGT990.12361711160502459No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC970.1211197962190645No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT880.1098818769822441No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG860.10738456159628398No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGACGT810.10114127313138375No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGA150.002212776870.0362519
CAACCAA150.002212776870.0362521
ACCAAGG150.002212776870.0362523
GCTAATG150.002212776870.0362527
CTAATGG150.002212776870.0362528
TTAATAC150.002218271669.992513
GCAACTC150.002218271669.9925135
TTATGCT150.002218271669.9925152
TCGGGCA150.002218271669.9925133
CATCTAC150.002218271669.992519
AGCGGGG150.002218271669.9925170
TCTACGA150.002218271669.9925111
CTTATGC150.002218271669.9925151
CTAGTAT150.002218271669.9925139
CTCGGGC150.002218271669.9925132
CTAAAAC150.002218271669.992511
TACGAGC150.002218271669.9925113
CTACGAG150.002218271669.9925112
CCGAGAC301.01290425E-769.9925114
ACATCTA150.002218271669.992518
Produced by FastQC (version 0.11.5)