FastQCFastQC Report
Wed 25 May 2016
SRR1779957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329994
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG296938.998042388649491No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG28830.8736522482226949No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC5860.17757898628459912No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG3370.10212306890428312No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC150.00222376269.9978832
GACCTAT150.00222376269.997881
GTACAGA252.388888E-455.9983026
CGTATTT200.00694433352.5063761
CACGTAC200.00694433352.5063739
CGACACG200.00694849852.49841336
ATCGTAC305.8697653E-446.67232525
CATCGTA305.8697653E-446.67232524
GACCCAT305.8741536E-446.6652531
CTCGGTG5100.045.9720344
CGGTGGT5100.045.9720346
TGGTCGC5200.045.76090649
GCCGTAT5150.045.532654
TCGCCGT5200.045.0879552
GATCTCG5300.044.91110641
GTCGCCG5300.044.8974951
TCGGTGG5300.044.23723645
ATCTCGG5400.044.0727442
CGTATCA5400.043.4311956
TCTCGGT5400.043.4246143
Produced by FastQC (version 0.11.5)