FastQCFastQC Report
Wed 25 May 2016
SRR1779957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329994
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT189465.741316508785008No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53641.6254840997109037No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG23640.7163766613938435No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC22450.6803153996739334No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT7460.22606471632817568RNA PCR Primer, Index 34 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT7430.2251556088898586No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC4490.1360630799347867No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCGG150.002223475470.0001539
CGAATGC301.0219992E-770.0001541
AACTCGA207.920168E-570.0001537
AGGGGGG23500.058.53204370
GGACCTA252.3867167E-456.008611
CAGATCT200.006943442452.508074
CACTCGC200.006947606352.50011444
GCCGTCT27500.052.05465746
CCGTCTT27450.052.0219747
CGTCTTC28350.051.9675548
TATGCCG28150.051.47435443
ATCTCGT28900.051.470737
CGTATGC28300.051.4488741
TCTCGTA29000.051.41390638
TCGTATG29000.051.41390640
CTCGTAT28800.051.28483239
GTCTTCT28500.051.20287749
ATGCCGT28700.050.9757244
CTTGAAA28300.050.9464557
TCTTCTG28600.050.90148550