FastQCFastQC Report
Wed 25 May 2016
SRR1779956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298904
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3236310.827222118138264No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG33241.1120627358616813No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC5900.19738779005968474No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG5170.1729652329845034No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT3510.11742900730669378No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCT200.006945190552.50351336
CCGTGCG200.00694978852.4947310
CAGTAGT352.084973E-549.9949841
CGGTGGT6700.041.7938446
GCCGTAT6700.041.7868554
TCGGTGG6600.041.36640545
CTCGGTG6650.041.0553844
TCGCCGT6700.040.74899352
TGGTCGC6750.040.4471549
CGCTTAG350.001253865940.00267419
CAGTTGT350.00125489839.9959871
GTCGCCG6850.039.8566851
AGATCTC6950.039.78683540
ATCTCGG6950.039.28320342
CGTATCA7050.039.22248556
TCTCGGT7000.039.0026143
CGCCGTA7000.039.0026153
CCGTATC7100.038.94626655
GATCTCG7100.038.94626641
GTAGATC7300.038.8382238