FastQCFastQC Report
Wed 25 May 2016
SRR1779956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298904
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT207486.941359098573455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60962.0394507935658273No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC25750.8614806091587934No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG22980.7688087145036534No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT8600.2877177956802184No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT7610.25459679361935605TruSeq Adapter, Index 10 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC4440.14854267590932205No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA150.002224301569.99197441
ATTCGGA150.002224301569.99197436
CGAATGC252.8377817E-669.99197441
AGGGGGG25350.061.15669370
AAACTCG352.970828E-759.99311436
CGCCTCG200.0069409952.51154729
GGGCTCG200.006945585452.502761
CACGGTT200.00695018352.49397746
ATATCGT407.471481E-752.49397737
ACGTATG200.00695018352.49397740
ACGGTTA200.00695018352.49397747
AAGGGGG30900.050.05898369
TATGCCG32950.049.5997743
GCCGTCT32850.049.53769346
CTTGAAA32750.049.4835257
CCGTCTT32550.049.2416647
AATCTCG33050.049.23792336
CGTATGC33200.049.22627641
CGTCTTC32500.049.2097448
TCGTATG33250.049.15225640
Produced by FastQC (version 0.11.5)