FastQCFastQC Report
Wed 25 May 2016
SRR1779955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145497
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3453223.73382269050221No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG40652.793872038598734No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT5160.35464648755644446No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC4680.3216561166209613No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG3520.24192938686021015No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG150.002221291469.997637
GAGAACT252.3804315E-456.01732616
TGATCAA252.3844813E-455.99807748
TGTTCCG200.006931405552.51624719
CATTGCT200.006940840752.4981957
GCTGCGT200.006940840752.4981961
TTAAGGT200.006940840752.498194
CTTTCGC200.006940840752.4981929
CCACGTT200.006940840752.4981937
GTTATTG200.006940840752.4981934
CTACTGG305.8633817E-446.6650629
TCGGTGG7400.045.876845
CTCGGTG7350.045.71271544
GCCGTAT7200.045.69287554
AAAAAGG4300.045.5798370
TCGCCGT7300.045.54638352
GTCGCCG7400.045.40384351
TGGTCGC7500.045.2651149
AGATCTC7450.045.09911740
TAGATCT7500.044.79846239
Produced by FastQC (version 0.11.5)