FastQCFastQC Report
Wed 25 May 2016
SRR1779955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145497
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2356316.194835632349807No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66814.591847254582569No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG25361.742991264424696No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC19821.3622273998776606No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT8750.6013869701780793No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC5080.3491480924005306No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT4970.34158779906114906RNA PCR Primer, Index 14 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC2660.18282163893413608No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2120.1457074716317175No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGA150.002223066369.983511
ATTCGCC150.002223066369.983545
ATGTCGC150.002223066369.983537
ATCGTAT301.0196709E-769.983539
ATATCGT353.6907295E-969.983537
AGGGGGG28800.064.6597770
AAGGGGG33200.056.09039769
TTCTAAG252.3868543E-455.986866
CAGAGCT252.3868543E-455.986818
GTCTTCT33500.054.96116349
TCTTCTG33600.054.90176450
CCGTCTT34300.054.88501747
GCCGTCT34650.054.7345746
TTCTGCT33150.054.69671252
CGTCTTC34050.054.69019748
ATGCCGT35200.054.575244
GTTACCA452.6793714E-854.4503331
CTTCTGC33750.054.4503351
TATGCCG35300.054.42059343
TGCCGTC35200.054.37638545