FastQCFastQC Report
Wed 25 May 2016
SRR1779954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246385
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4041116.401566653814154No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG38261.5528542727844632No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC4650.18872902165310387No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT4450.18061164437770155No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG4350.1765529557400004No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG150.002222209770.0048751
GACCCAT150.002222209770.0048751
GTTACGG150.002224000269.9906622
GGTTACG150.002224000269.9906621
TGCATCG200.006943672452.5036545
GTTCGAA200.006943672452.50365438
ATATACG200.006943672452.5036542
TGTTCGA200.006943672452.50365437
CGAATTC200.006943672452.50365441
CGAGGGG200.00694925152.49299625
GCGGATA200.00694925152.49299613
CCGTGCG200.00695483352.4823410
GCGTTTA200.00695483352.4823412
GCCGTAT7200.047.1560654
CGGTGGT7350.046.66991846
ATCTCGG7200.046.66991442
CTTGGAT305.868345E-446.6699143
GAGCCGT305.868345E-446.66991434
TCGCCGT7300.046.51008652
AGATCTC7300.046.51008640
Produced by FastQC (version 0.11.5)