FastQCFastQC Report
Wed 25 May 2016
SRR1779954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246385
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT160256.504048541916108No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68062.7623434868194083No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT30271.2285650506321406No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG12430.504494997666254No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC9420.3823284696714491No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT7420.30115469691742597No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT5810.23580980985043734No Hit
CATATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT3920.15910059459788542No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT3870.15707125027903485No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG3720.1509832173224831No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT3460.1404306268644601No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT3340.1355602004992187No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT2980.12094892140349453No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT2860.11607849503825314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA150.002219348270.0276229
AAACGGT150.002222926469.9991824
CGTTTTC150.002222926469.9991818
CAGCCGT150.00222471769.984981
CGTCACA150.00222471769.984981
CGAATTA401.0642907E-861.23686248
AGCCGTC750.060.66596245
TAAGACG352.9665534E-759.99930643
AGGGGGG24150.059.27467370
ACGTATG308.361216E-658.33265340
TCTCGGA850.057.6463938
TCGGATG553.6379788E-1157.27206440
TCGTATG23800.057.2052240
TCTCGTA25550.056.9856438
CGTATGC23400.056.83694541
CTCGTAT24950.056.8129739
CATCTCG27350.056.56241636
ATCTCGT25900.056.48583637
TTAAGCA2550.056.27385752
GCCGTAT3300.056.21146846
Produced by FastQC (version 0.11.5)