FastQCFastQC Report
Wed 25 May 2016
SRR1779953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148509
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2951319.872869657731183No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG26951.8147048327037416No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC4710.3171524958083349No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT2750.18517396252079No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG2510.16901332579170286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACA200.006935426552.509094
TAACCGC200.006935426552.5090922
CGGGTGG200.006944672752.491419
CCAGGAA200.006944672752.4914119
CTGGGCG200.006944672752.4914114
ATCTCGG4900.049.29425442
CTCGGTG4900.049.27765744
TCGCCGT4850.049.0641452
AGATCTC4950.048.79633340
CGCCGTA4900.048.5798453
GCCGTAT4900.048.5798454
CGGTGGT5050.048.50691646
TCGGTGG5050.048.50691645
GATCTCG5000.048.3083741
CGTATCA4950.048.08913856
GTCGCCG5100.048.03135751
TGGTCGC5100.048.03135749
TCTCGGT5050.047.83006743
GTATCAT5000.047.60824657
TAGATCT5100.047.3611439
Produced by FastQC (version 0.11.5)