FastQCFastQC Report
Wed 25 May 2016
SRR1779953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148509
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1834212.350766620204837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48433.2610818199570395No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG22761.532567049808429No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC17551.1817465608144961No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT7520.5063666175113967No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT7300.49155270050973343No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT6410.4316236726393687Illumina PCR Primer Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC4320.2908914611235683No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT2880.19392764074904553No Hit
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2680.18046044347480625No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATGA150.002217816270.0259428
TGGCACA150.002220782770.0023652
CGAATGC207.904613E-570.0023641
CGAATCT150.002220782770.0023631
ACGACCG150.002220782770.0023637
CGTATAG150.00222375269.9787917
CGCTATT150.00222375269.9787957
AGGGGGG23250.062.0444970
GTGGTTC308.342682E-658.335331
ACGTATG252.3838399E-456.0018940
ACAGACG252.3878121E-455.9830328
AAGGGGG27450.052.678969
GTGACGA200.006930011352.51945534
GTGCAGC200.00693925452.50176650
GACAGGA200.00693925452.5017661
CGCGGTC200.00693925452.50176632
CCGATGG200.00693925452.50176644
CGACCGG200.00693925452.50176638
TGACGAT200.00693925452.50176635
TGTGACG200.00693925452.50176633
Produced by FastQC (version 0.11.5)